Exogenous administration of adenosine enhanced glucose uptake in canine hind limb at rest and during contraction

Abstract

Background: Glucosc metabolism increases during contraction of skeletal muscle and can be influenced by the endogenous adenosine. However, the role of exogenous adenosine in regulating glucose uptake at rest or during contraction has not been elucidated in dogs. We studied the effects of exogenous adenosine on glucose uptake in canine hind limb at rest and during contraction. Methods: The study was carried out using thirty (30) fasted and anaesthetized male dogs divided into six groups (5dogs/group). Groups I (control) and 11 received normal saline (0.1 ml/kg) at rest and during contraction of hind limb respectively. Group III received adenosine (0.1, 0.5 and I mg/kg) at rest. Group IV were treated with adenosine (I mg/kg) during contraction. Groups V and IV were protracted with caffeine (6mg/kg) and infused with adenosine (I mg/kg) for thirty minutes at rest and during contraction of the hind limb respectively. Blood glucose was measured by glucose oxidase method. Arteria venous (A-V) glucose and venous blood flow (VBF) were measured; hind limb glucose uptake (HGU) was calculated as the product of A-V glucose and VBF. Results: The results showed that exogenously administered adenosine significantly