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Title: | Anti-inflammatory, antinociceptive and total polyphcnolic content of hydroethanolic extract of Ocimum gratissimum L. leaves |
Authors: | Ajayi, AM Tanayen, JK Ezeonwumelu, JOC Dare, S Okpanachi, A Adzu, B Ademowo, OG |
Keywords: | hydroethanolic erythrocytes carragcenan Ocimum gratissimum |
Issue Date: | Sep-2014 |
Publisher: | COLLEGE OF MEDICINE |
Citation: | Afr J Med. med. Sci. (Sept.. 2014) 43, Suppl 215-22 |
Abstract: | Background: Ocimum gratissimum has been reported in several ethnopharmacological surveys as a plant readily accessible to the communities and widely used with a lot of therapeutic potentials. In this study, we aimed to experimentally evaluate the anti-inflammatory effects of hydro-ethanol c extract in animal models of inflammation and nociception and membrane stabilization assay Method: 0 gratissimum leaves hydroethanolic extract was subjected to phytochemical screening and spectrophotometry quantification of polyphenolics. The extract was investigated for anti-inflammatory effects in carrageenan -induced paw oedema and cotton pellet - induced granuloma in rats. The antinociceptive effects were investigated in acetic acid -induced writhing in mice and formalin testing rats. Animals were randomly divided into groups; negative control, extract treated (200 -800 mg/kg) and indomethacin (10 mg/kg) standard reference groups. In- vitro anti-inflammatory activity was performed by testing for membrane stability in heat/hypotonic solution -induced rat erythrocytes destabilization assay. Result: Phytochemical screening revealed presence of saponins, tannins, alkaloids, flavonoids, terpenoids, and cardcnolidcs. Quantification of the polyphenolic content revealed the presence of appreciable quantities of phenolics and flavonoids. Carragccnan-induced paw edema, cotton-pellet granuloma, acetic acid -induced writhing and formalin induced paw licking tests showed that hydroethanolic extract of O. gratissimum possess anti-inflammatory and anti-nociceptive effects. The extract did not induce gastric lesion formation in stomach of cotton-pellet granuloma rats. The extract was more efficient at reducing membrane destabilization than indomethacin in the membrane stability assay |
Description: | Article |
URI: | http://adhlui.com.ui.edu.ng/jspui/handle/123456789/2825 |
ISSN: | 1116-4077 |
Appears in Collections: | African Journal of Medicine and Medical Sciences |
Files in This Item:
File | Description | Size | Format | |
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Ajayi et al_Anti_2014.pdf | Article | 19.54 MB | Adobe PDF | View/Open |
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