Please use this identifier to cite or link to this item: http://adhlui.com.ui.edu.ng/jspui/handle/123456789/2825
Title: Anti-inflammatory, antinociceptive and total polyphcnolic content of hydroethanolic extract of Ocimum gratissimum L. leaves
Authors: Ajayi, AM
Tanayen, JK
Ezeonwumelu, JOC
Dare, S
Okpanachi, A
Adzu, B
Ademowo, OG
Keywords: hydroethanolic
erythrocytes
carragcenan
Ocimum gratissimum
Issue Date: Sep-2014
Publisher: COLLEGE OF MEDICINE
Citation: Afr J Med. med. Sci. (Sept.. 2014) 43, Suppl 215-22
Abstract: Background: Ocimum gratissimum has been reported in several ethnopharmacological surveys as a plant readily accessible to the communities and widely used with a lot of therapeutic potentials. In this study, we aimed to experimentally evaluate the anti-inflammatory effects of hydro-ethanol c extract in animal models of inflammation and nociception and membrane stabilization assay Method: 0 gratissimum leaves hydroethanolic extract was subjected to phytochemical screening and spectrophotometry quantification of polyphenolics. The extract was investigated for anti-inflammatory effects in carrageenan -induced paw oedema and cotton pellet - induced granuloma in rats. The antinociceptive effects were investigated in acetic acid -induced writhing in mice and formalin testing rats. Animals were randomly divided into groups; negative control, extract treated (200 -800 mg/kg) and indomethacin (10 mg/kg) standard reference groups. In- vitro anti-inflammatory activity was performed by testing for membrane stability in heat/hypotonic solution -induced rat erythrocytes destabilization assay. Result: Phytochemical screening revealed presence of saponins, tannins, alkaloids, flavonoids, terpenoids, and cardcnolidcs. Quantification of the polyphenolic content revealed the presence of appreciable quantities of phenolics and flavonoids. Carragccnan-induced paw edema, cotton-pellet granuloma, acetic acid -induced writhing and formalin induced paw licking tests showed that hydroethanolic extract of O. gratissimum possess anti-inflammatory and anti-nociceptive effects. The extract did not induce gastric lesion formation in stomach of cotton-pellet granuloma rats. The extract was more efficient at reducing membrane destabilization than indomethacin in the membrane stability assay
Description: Article
URI: http://adhlui.com.ui.edu.ng/jspui/handle/123456789/2825
ISSN: 1116-4077
Appears in Collections:African Journal of Medicine and Medical Sciences

Files in This Item:
File Description SizeFormat 
Ajayi et al_Anti_2014.pdfArticle19.54 MBAdobe PDFView/Open


Items in COMUI (ADHL) are protected by copyright, with all rights reserved, unless otherwise indicated.