In vitro inhibition of glucose transport across the intestinal membrane of mice exposed to trivalcnt chromium.

Abstract

Background: Trivalent chromium (Cr34) supplementation has been used in the management t of type-2 diabetes mellitus and the small intestine plays significant role in glucose homeostasis. However, there is dearth of information on the glucose absorption ability of normal gut during Cr34 exposure. In this study, we investigated the effect of Cr34 exposure in the absorption of glucose e in the normal gut. Methodology: Thirty male slc:ddY mice (26.2 ±1.1g) were randomly and equally y assigned to three groups: Group 1 (control) received drinking water while animals in groups 2 and 3 received 10 and 100 ppm Cr34 t respectively for 12-week s through drinking water. Thereafter, they were sacrificed and their intestines excised, rinsed with ice-cold Ringer solution (RS) and nine everted-sacs were made, with addition of 200 µL RS. The sacs were incubated for 1 hour in 5 mL glucose-free RS and glucose concentrations were determined by spectrophotometry. Transmural potential change (P∆t) was assessed using the short-circuit currents. Data were analysed by one-way ANOVA and p< 0.05 was considered significant. Results: A significant decrease in glucose concentration at the distal jejunum of the serosa in test groups compared with control was observed. The mucosa glucose concentration was s elevated at the same region compared with control. The P∆t across the membrane reduced significantly at both the distal jejunum and ileum of Cr34 exposed groups compared with control. Conclusion: It may be concluded that Cr34 exposure reduced intestinal glucose transport which might probably be a mechanism explore d during management of diabetes.