Please use this identifier to cite or link to this item: http://adhlui.com.ui.edu.ng/jspui/handle/123456789/3904
Title: Properties of lactase produced by enteropathogenic Escherichia coli from diarrhoeic children
Authors: OLUSANYA, O.
OLUTIOLA, P. O.
Keywords: Lactase
Escherichia coli
diarrhoeic children
Issue Date: 1989
Publisher: COLLEGE OF MEDICINE, UNIVERSITY OF IBADAN, NIGERIA
Citation: Afr J Med Med Sci 1989, 18(3):163-168
Abstract: The quantity of lactase produced by enteropathogenic Escherichia coli (EPEC) was significantly higher (P< 0.01) than that produced by non-EPEC. The enzyme production was induced by lactose but repressed by glucose and galactose. The lactase from EPEC which was partially purified by ammonium sulphate precipitation and gel permeation chromatography had a molecular weight of 56 kD and apparent Km of approximately 2.78 mM for lactose. The lactase exhibited optimum activity at pH7.0 at 40°C and was stimulated by Mg2+, Mn2+, Na+ and inhibited by Ba2+, Ca+, Cu2+. EDTA, iodo acetic acid (IAA) and Hg2+ and U2+ ions. The higher production of lactase by EPEC may be linked to its pathogenic role in childhood diarrhea.
Description: Article
URI: http://adhlui.com.ui.edu.ng/jspui/handle/123456789/3904
ISSN: 1116-4077
Appears in Collections:African Journal of Medicine and Medical Sciences

Files in This Item:
File Description SizeFormat 
Olusanya_Olutiola_Properties_1989.pdfArticle7.3 MBAdobe PDFView/Open


Items in COMUI (ADHL) are protected by copyright, with all rights reserved, unless otherwise indicated.