Please use this identifier to cite or link to this item:
http://adhlui.com.ui.edu.ng/jspui/handle/123456789/3904
Title: | Properties of lactase produced by enteropathogenic Escherichia coli from diarrhoeic children |
Authors: | OLUSANYA, O. OLUTIOLA, P. O. |
Keywords: | Lactase Escherichia coli diarrhoeic children |
Issue Date: | 1989 |
Publisher: | COLLEGE OF MEDICINE, UNIVERSITY OF IBADAN, NIGERIA |
Citation: | Afr J Med Med Sci 1989, 18(3):163-168 |
Abstract: | The quantity of lactase produced by enteropathogenic Escherichia coli (EPEC) was significantly higher (P< 0.01) than that produced by non-EPEC. The enzyme production was induced by lactose but repressed by glucose and galactose. The lactase from EPEC which was partially purified by ammonium sulphate precipitation and gel permeation chromatography had a molecular weight of 56 kD and apparent Km of approximately 2.78 mM for lactose. The lactase exhibited optimum activity at pH7.0 at 40°C and was stimulated by Mg2+, Mn2+, Na+ and inhibited by Ba2+, Ca+, Cu2+. EDTA, iodo acetic acid (IAA) and Hg2+ and U2+ ions. The higher production of lactase by EPEC may be linked to its pathogenic role in childhood diarrhea. |
Description: | Article |
URI: | http://adhlui.com.ui.edu.ng/jspui/handle/123456789/3904 |
ISSN: | 1116-4077 |
Appears in Collections: | African Journal of Medicine and Medical Sciences |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Olusanya_Olutiola_Properties_1989.pdf | Article | 7.3 MB | Adobe PDF | View/Open |
Items in COMUI (ADHL) are protected by copyright, with all rights reserved, unless otherwise indicated.