Effect of taurine and caffeine on plasma c-reactive protein and calcium in Wistar rats

Abstract

Background: Caffeine is a component of several beverages such as coffee and tea. It has been shown to possess psychoactive properties because it increases alertness, energy and ability to concentrate at moderate doses. Taurine on the other hand, is an amino acid which has the capacity to promote neural development, osmoregulation and neuroprotection. There is paucity of information on the effect of the combined administration of taurine and caffeine on C-reactive protein (CRP) - a marker of inflammation and plasma calcium level in rats. Aim: The present study was designed to investigate the effects of combined taurine and caffeine on the plasma level of CRP, Ca 2' as well as the effect of nifedipine on calcium level. Method: Fifty four rats weighing 120-140 g were used for these studies. The animals were divided into nine groups consisting of six animals each. Group 1 was treated with 10 ml/kg of normal saline. Groups 2 and 3 were given 100 mg/kg and 200 mg/kg of taurine respectively, groups 4 and 5 received 7.5 mg/ kg and 15 mg/kg of caffeine respectively while group 6 was administered taurine (200 mg/kg) and caffeine (15 mg/kg), groups 7 and 8 were treated with taurine (200 mg/kg) plus nifedipine (10 mg/kg) and taurine (200 mg/kg)plus furosemide (20 mg/kg) respectively while group 9 was given taurine plu caffeine plus nifedipine plus furosemide. Treatment was done once daily for 21 days and blood was finally collected via cardiac puncture for the assay of CRP and calcium while the animals were under anesthesia. Results: The results showed that CRP was significantly decreased in five of the treated groups compared with the control with the exception of the group treated with taurine alone (Group 2), and that treated with combined taurine and caffeine (Group 6). The Ca2' level of groups treated with caffeine (11.70 ± 0.29 mg/dL) and taurine with caffeine (11.64 ±0.15 mg/dL) were significantly (p<0.05) increased compared with the control (10.70 ± 0.29 mg/dL).However, treatment with taurine and nifedipine (Group 7) led to significant (p<0.05) reduction in plasma Ca2' level. Conclusion: The results have shown that combined caffeine and taurine can boost plasma calcium level and decrease plasma CRP level. Moreover, taurine combined with nifedipine but not furosemide can act synergistically to lower both plasma Ca2' and CRP levels, a result which may have implication for the treatment of hypertension