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http://adhlui.com.ui.edu.ng/jspui/handle/123456789/1996
Title: | A modified medium for the in vitro cultivation of trypanosomes |
Authors: | OTIGBUO, I. N OYERINDE, J. P. O |
Keywords: | Parasites cultivation trypanosomes |
Issue Date: | Dec-1993 |
Publisher: | Spectrum Books Limited |
Citation: | Afr. J. Med med Sci. (1993) 22, 63-71 |
Abstract: | A modified medium (ME-99) for the in vitro cultivation of trypanosomes was developed and evaluated in our laboratory. The medium comprised of Eagle MEM as a base and various components of medium 199 not found in MEM. Trypanosoma brucei brucei, T. b. gambiense and T. vivax were grown in ME-99 and various other media combinations such as MEM/RPMI 1640, MEM/BCM, MEM/199, RPMI 1640/BCM, RPMI 1640/199, BCM/199 for comparison. Both growth and infectivity of these parasites were compared in these media. Parasites grew better and maintained their infectivity for longer periods in ME-99 than in any other medium or combination of media. |
Description: | Article |
URI: | http://adhlui.com.ui.edu.ng/jspui/handle/123456789/1996 |
ISSN: | 1116-4077 |
Appears in Collections: | African Journal of Medicine and Medical Sciences |
Files in This Item:
File | Description | Size | Format | |
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Otigbuo_Oyerinde_Modified_1993.pdf | Article | 11.85 MB | Adobe PDF | View/Open |
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