http://adhlui.com.ui.edu.ng/jspui/handle/123456789/72 Theses in Obstetrics and Gynaecology 2026-02-24T21:39:30Z http://adhlui.com.ui.edu.ng/jspui/handle/123456789/453 Title: CIRCULATING TUMOUR ASSOCIATED ANTIGENS AS AID TO EARLY DIAGNOSIS OF CARCINOMA OF CERVIX Authors: ADELUSI, BABATUNDE Abstract: Earlier studies by this author in 1976 provides evidence of an association between an infective, sexually transmitted agent, the Herpes Simplex Virus Type-2 (HSV-2) and human carcinoma of the cervix, irrespective of geographic location or ethnic origin of the individuals. Similarly, HSV-2 related antigens were demonstrated by the indirect immuno-fluorescent technique in desquamated cells of carcinoma of the cervix tissues from tumour bearing patients. To highlight the potentials of the detection of tumour antigens in tumour-bearing patients as aid to early diagnosis of the cancer, isolation and purification of the Tumour Associated Antigens (TAA) of human cancer of the cervix were attempted by immunological and physiological procedures in this study. The antigens preparations, one of the Soluble Antigens (SA), and the other, the Membrane bound Antigens (MBA) were prepared from a pool of cancer of the cervix (CaCx) tissues. The soluble antigens were extracted by homogenization in Earle's Balanced Salt Solution (EBSS) containing antiboitics, and the membrane-bound antigens were solublised by 3-molar potassium chloride (3 Molar KCl). For isolation and purification of TAA, procedures for the purification of proteins were explored. These include physico-chemical stepwise ammonium sulphate fractionation, sephadex ion exchange chromatography, sephacryl-5-200 gel filtration, and affinity chromatography to eliminate the normal tissue components of the antigens fractions. Hyperimmune rabbit sera were then prepared against the partially purified TAA. Sensitivity of the test method was low, the specificity was high, and could provide a means of early diagnosis of neoplastic changes in the cervix. The detection of Circulating-TAA as tumour markers in sera of patients, may some day become routine, and thus make earlier diagnosis of cancer possible. Indications are that immune-diagnostic procedures can be designed in ways that are reproducible, simple and reliable. Such serological tests would extend our present ability for detection and monitoring of malignancies. Description: A Thesis in the Department of Obstetrics and Gynaecology submitted to the Faculty of Clinical Sciences and Dentistry, College of Medicine in partial fulfillment of the requirement for the Degree of Doctor of Medicine of the University of Ibadan. 1982-10-01T00:00:00Z http://adhlui.com.ui.edu.ng/jspui/handle/123456789/89 Title: HERPES TYPE-2 VIRUS AND CARCINOMA OF CERVIX UTERI IN NIGERIANS: IMMUNOVIROLOGICAL STUDIES Authors: ADELUSI, BABATUNDE Abstract: In the search for etiology of carcinoma of the cervix uteri, various factors have been implicated by various workers. The disease has been shown to have a venereal origin, following on its significant association with coital characteristics. These include early initiation into hetreo-sexual acts and frequency of coitus, multiplicity of coital partners, multiparity, low socio-economic standards, venereal diseases and circumcision. Extensive epidemiologic studies have indicated that a “venereally transmitted factor” might be responsible for the induction of the squamoua cell variety of the malignant disease. Recently, a strain of Herpes simplox virus, antigenically distinct from the strain commonly associated with oral lesions, and designated Genital Herpes or Herpes Type -2 (HT-2) virus, was shown to be venereally transmitted, and might have oncogenic potentialities on the cervix. At the time the present study were contemplated, little was documented about the clinical and histopathologic presentation of carcinoma of the cervix in Ibadan. There was also no knowledge of the precise relationship, if any, of the virus to carcinoma of the cervix uteri in Ibadan. It was clear however; as a result of the work of Edington and Vaclean (1965) that carcinoma of the cervix uteri is very common in Ibadan, where it was shown to form the commonest female malignancy. Clinical, cytology, histopathologic and immune-virologic studies were undertaken to see if indeed carcinoma of the cervix uteri has a venereal origin, and to ascertain whether or not there is any association between the malignancy and HT-2 virus infection in Ibadan. In addition, sero-epidemiologic studies were also undertaken to determine the prevalence of HT-2 virus antibodies in various sectors of the population. Evidence was provided to show that coital practice was a significant correlate of carcinoma of the cervix in Ibadan. Furthermore, Immuno-fluorescence and complement fixation tests were two parameters used to provided evidence that Herpes Type-2 virus is associated with the disease, in that carcinoma of the cervix patients possessed significantly higher levels of antibody against HT-2 virus as compared with patients having extra-cervical pelvic, and extra-pelvic malignancies and healthy controls. In addition, HT-2 virus antigens were detected by immunofluorence tests in the oxfoliative cervical cells from all patients with carcinoma of the cervix, whereas no such virus antigens were found oxfoliated cervical cells from healthy controls. It was concluded, that the observed association between HT-2 virus and carcinoma of the cervix in agreement with other studies, and the fact that the virus was not associated with other extra-cervical malignancies in this environment, indicate a significant relationship. Even though this may not necessarily be an etiologic one, the precise relationship of the virus and malignancy would have to await further investigation. Description: A THESIS IN THE DEPARTMENT OF OBSTETRICS AND GYNECOLOGY SUBMITTED TO THE COLLEGE OF MEDICINE IN PARTIAL FULFILLMENT OF THE DEGREE OF DOCTOR OF PHILOSOPHY OF THE UNIVERSITY OF IBADAN, IBADAN, NIGERIA. 1976-03-01T00:00:00Z